ABSTRACT
Abstract INTRODUCTION: Genetic polymorphisms define the cytokine production leading to susceptibility or resistance to diseases. We studied the cytokine polymorphism in the development of tegumentary leishmaniasis (TL). METHODS: Genotyping of TNF-α, TGF-β1, IFN-γ, IL-6, and IL-10 were performed by polymerase chain reaction assay. RESULTS: G and C alleles of TGF- β1 (codon 25) were the most common in controls and patients, respectively. G/G was the most frequent genotype in controls, and G/C and C/C in patients. CONCLUSIONS: G/G genotype of codon 25 in TGF-β1 appeared to confer resistance, and G/C and C/C genotypes, susceptibility to TL in this population.
Subject(s)
Humans , Animals , Male , Female , Snake Bites/diagnosis , Snakes/anatomy & histology , Snake Bites/epidemiology , Snakes/classification , Snakes/physiology , Brazil/epidemiology , Colubridae , Diagnostic ErrorsABSTRACT
OBJECTIVE: To determine the prevalence of the Torque teno virus in healthy donors in the northern and northwestern regions of the state of Paraná, southern Brazil.METHODS: The Torque teno virus was detected by a nested polymerase chain reaction using a set of oligoprimers for the N22 region.RESULTS: The prevalence of the virus was 69% in 551 healthy blood donors in southern Brazil. There was no statistically significant difference between the presence of the virus and the variables gender, ethnicity and marital status. There was significant difference in the prevalence of the virus regarding the age of the donors (p-value = 0.024) with a higher incidence (74.7%) in 18- to 24-year-old donors.CONCLUSION: A high prevalence of Torque teno virus was observed in the population studied. Further studies are needed to elucidate the routes of contamination and the clinical implications of the virus in the healthy population.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Virology , Blood Donors , Polymerase Chain Reaction , Torque teno virus , AnelloviridaeABSTRACT
Objetivou-se investigar a soroprevalência do Citomegalovírus Humano em dialíticos e transplantados renais e a associação com a hemotransfusão e o sexo. Trata-se de estudo transversal, realizado nos anos de 2011 e 2012, no município de Maringá, PA, Brasil. A população estudada foi composta por 203 pacientes dialíticos e 53 transplantados renais e a identificação viral foi realizada por meio de técnica sorológica. Encontrou-se 96% (195) de soropositividade para o anti-HCMV-IgG em dialíticos e 100% nos transplantados; 5% (10) de soropositividade para o anti-HCMV-IgM em dialíticos e 37,7% (20) em transplantados. Realizando o teste exato de Fischer, não houve associação significante entre a soropositividade do anti-HCMV com a hemotransfusão e o gênero. A grande parcela de infectados sugere a importância do cuidado de enfermagem para evitar a infecção cruzada durante os procedimentos de rotina...
The aim of this study was at investigating the seroprevalence of Human Cytomegalovirus in dialysis and kidney transplantedpatients and the association with hemodialysis and sex. It is a cross sectional study made in 2011 and 2012 in the countyof Maringá, PR, Brazil. The studied population consisted of 203 dialysis patients and 53 kidney transplanted patients andthe viral identification was made through serological technique. The study found 96% (195) seropositivity for anti-HCMVIgG in dialysis patients and 100% in the transplanted ones; 5% (10) seropositivity for anti-HCMV-IgM in dialysis patientsand 37.7% (20) in transplanted ones. Performing Fishers exact test, there was no significant association between theseroprevalence of anti-HCMV with hemodialysis and gender. A large number of infected patients suggest the importance ofnursing care to avoid cross-infection during routine procedures...
El objetivo fue investigar la seroprevalencia de Citomegalovirus Humano en dialíticos y trasplantados renales y la asociacióncon la transfusión de sangre y el sexo. Estudio transversal, llevado a cabo de 2011 a 2012, en Maringá, PR, Brasil. La poblaciónfue compuesta de 203 pacientes dialíticos y 53 trasplantados renales y la identificación viral se realizó mediante la técnicaserológica. Se encontró 96% (195) de seropositividad para anti-HCMV-IgG en dialíticos y 100% en los trasplantados; 5%(10) de seropositividad para anti-HCMV-IgM en dialíticos y 37,7% (20) en trasplantados. Realizándose la prueba exacta deFisher, no hubo asociación significativa entre la seropositividad del anti-HCMV con la transfusión de sangre y género. A lagran parte de los infectados se señala la importancia de la atención de enfermería para prevenir la infección cruzada durantelos procedimientos de rutina...
Subject(s)
Cytomegalovirus , Renal Insufficiency, ChronicABSTRACT
The profile of patients undergoing haemodialysis in the dialysis unit of Hospital Santa Casa de Maringá, Maringá PR Brazil, is provided. A questionnaire on social and economic data and underlying diseases prior to the Chronic Kidney Disease (CKD) identified the patients' profile. The project was approved by the Ethics Committee of the institution. Eighty-three patients, with 54.21% males, were interviewed. Age bracket ranged between 20 and 59 years in 65.06% of patients. Only 27.71% maintained jobs after the diagnosis and the start of treatment; 63.86% had an average personal income between 1 and 3 minimum wages; 63.85% did not practice any physical activity. Moreover, 53.01% belonged to the European-Brazilian white group; 20.48% to the Afro-Brazilian brown group; 19.28% to the Afro-Brazilian Negro group; 6.02% to other ethnic groups. Further, 85.54% patients reported having an underlying disease prior to the CKD, namely, 61.45% were hypertensive; 31.33% were diabetics and 20.48% had other diseases. Results show the need of a greater attention to these patients' health care to reduce the negative impacts related to the chronic disease focused.
Este estudo teve como objetivo identificar o perfil dos pacientes em tratamento hemodialítico do setor de diálise do Hospital Santa Casa de Maringá - Paraná, Brasil. Para a identificação do perfil destes pacientes foi utilizado um questionário contendo questões que abrangem dados socioeconômicos e doenças de base anteriores à Insuficiência Renal Crônica (IRC). O projeto foi aprovado pelo Comitê de Ética em Pesquisa da instituição. Entre os 83 pacientes entrevistados, 54,21% eram do sexo masculino. A faixa etária variou de 20 a 59 anos para 65,06% dos pacientes. Somente 27,71% continuaram trabalhando após o diagnóstico e início do tratamento. A renda pessoal média ficou entre um e três salários mínimos para 63,86% dos indivíduos. A atividade física está ausente em 63,85%. Quanto à etnia, foi verificado que 53,01% eram brasileiros descendentes de europeus/brancos, 20,48% brasileiros afrodescendentes/pardos, 19,28% brasileiros afrodescendentes/negros e 6,02% declararam como pertencentes à outras etnias. Entre os pacientes, 85,54% declararam possuir outra doença de base anterior à IRC sendo: 61,45% hipertensão arterial, 31,33% diabetes e 20,48% outras doenças. Estes resultados indicam a necessidade de uma melhor atenção ao cuidado da saúde desses pacientes para a redução dos impactos negativos relacionados à cronicidade da doença.